Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
Endothelial nitric oxide synthase in rat brain is downregulated by sub-chronic antidepressant treatment.
Psychopharmacology (Berl)
. 2017 Jun;234(11):1663-1669. doi: 10.1007/s00213-017-4567-z. Epub 2017 Mar 8.
Endothelial nitric oxide synthase in rat brain is downregulated by sub-chronic antidepressant treatment
Yuta Yoshino 1, Shinichiro Ochi 1, Kiyohiro Yamazaki 1, Shunsuke Nakata 1, Jun-Ichi Iga 2, Shu-Ichi Ueno 1
Affiliations expand
PMID: 28275829 DOI: 10.1007/s00213-017-4567-z
Abstract
Background: Nitric oxide (NO) is a neurotransmitter that may be related to major depressive disorder (MDD) because the selective neuronal NO synthase (NOS) inhibitor, 7-nitroindazole, induces a dose-dependent antidepressant-like effect. NO modulates major neurotransmitters involved in the neurobiology of MDD, such as norepinephrine, serotonin, dopamine, and glutamate. In this study, we investigated the effects of antidepressants as NO modulators in acute and sub-chronic treatments.
Methods: Rats were injected with the SSRI paroxetine (PAR, 10 mg/kg), the SNRI milnacipran (MIL, 30 mg/kg), or the NaSSA mirtazapine (MIR, 10 mg/kg) for acute (1 h) or sub-chronic (3 weeks) treatment prior to analysis of nine brain regions (frontal cortex, temporal cortex, striatum, thalamus, hippocampus, midbrain, pons, cerebellum, and olfactory bulb). The mRNA expression levels of three NOS subtypes (neuronal: nNOS, inducible: iNOS, and endothelial: eNOS) were analyzed using real-time PCR with Taqman probes.
Results: Acute MIR treatment significantly increased nNOS mRNA expression in the hippocampus, midbrain, cerebellum and olfactory bulb, and iNOS mRNA expression in the frontal cortex and midbrain. Acute PAR and MIR treatments significantly increased eNOS mRNA expression in most brain regions. Conversely, sub-chronic treatment with all types of antidepressants resulted in significant decreases of eNOS mRNA expression in most brain regions.
Conclusions: Sub-chronic treatment with the three types of antidepressants consistently decreased eNOS mRNA expression levels in the rat brain. These effects may be associated with the involvement of the NO system in the mechanism of action of antidepressants.
Keywords: Antidepressant; Brain; Depression; Nitric oxide; Nitric oxide synthase; Rat; mRNA expression.
Psychopharmacology (Berl)
. 2017 Jun;234(11):1663-1669. doi: 10.1007/s00213-017-4567-z. Epub 2017 Mar 8.
Endothelial nitric oxide synthase in rat brain is downregulated by sub-chronic antidepressant treatment
Yuta Yoshino 1, Shinichiro Ochi 1, Kiyohiro Yamazaki 1, Shunsuke Nakata 1, Jun-Ichi Iga 2, Shu-Ichi Ueno 1
Affiliations expand
PMID: 28275829 DOI: 10.1007/s00213-017-4567-z
Abstract
Background: Nitric oxide (NO) is a neurotransmitter that may be related to major depressive disorder (MDD) because the selective neuronal NO synthase (NOS) inhibitor, 7-nitroindazole, induces a dose-dependent antidepressant-like effect. NO modulates major neurotransmitters involved in the neurobiology of MDD, such as norepinephrine, serotonin, dopamine, and glutamate. In this study, we investigated the effects of antidepressants as NO modulators in acute and sub-chronic treatments.
Methods: Rats were injected with the SSRI paroxetine (PAR, 10 mg/kg), the SNRI milnacipran (MIL, 30 mg/kg), or the NaSSA mirtazapine (MIR, 10 mg/kg) for acute (1 h) or sub-chronic (3 weeks) treatment prior to analysis of nine brain regions (frontal cortex, temporal cortex, striatum, thalamus, hippocampus, midbrain, pons, cerebellum, and olfactory bulb). The mRNA expression levels of three NOS subtypes (neuronal: nNOS, inducible: iNOS, and endothelial: eNOS) were analyzed using real-time PCR with Taqman probes.
Results: Acute MIR treatment significantly increased nNOS mRNA expression in the hippocampus, midbrain, cerebellum and olfactory bulb, and iNOS mRNA expression in the frontal cortex and midbrain. Acute PAR and MIR treatments significantly increased eNOS mRNA expression in most brain regions. Conversely, sub-chronic treatment with all types of antidepressants resulted in significant decreases of eNOS mRNA expression in most brain regions.
Conclusions: Sub-chronic treatment with the three types of antidepressants consistently decreased eNOS mRNA expression levels in the rat brain. These effects may be associated with the involvement of the NO system in the mechanism of action of antidepressants.
Keywords: Antidepressant; Brain; Depression; Nitric oxide; Nitric oxide synthase; Rat; mRNA expression.
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
Hum…
- Acute SSRI dose improved NO
- Chronic SSRI dose decreased NO
- Acute SSRI dose improved NO
- Chronic SSRI dose decreased NO
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
I started with this stack on friday (without 9 me bc) and did not notice any changes yet.
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
Well it takes a minimum of ~1 week to notice any differences, average is ~2 weeks, to notice any difference.flexstar13 wrote: ↑Mon Oct 11, 2021 4:00 pm I started with this stack on friday (without 9 me bc) and did not notice any changes yet.
A small percentage (maybe 4%) notice benefits to sexual function on the 3rd or 4th day. You've barely been on it 3 days, yet.
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
@Area1255_2021 - Do you have any explanation for why the only existing 3 positive reviews of your stack appeared within 6 minutes of each other, 5 days before you published your stack on your blog?Area1255_2021 wrote: ↑Mon Sep 13, 2021 1:23 pmThank You for linking these.facad2 wrote: ↑Mon Sep 13, 2021 12:07 pmThe comments were made here: https://area1255.blogspot.com/2014/11/i ... a.html?m=1
The stack post was made here: https://area1255.blogspot.com/2021/09/n ... d.html?m=1
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
Because I had a JavaScript program (script) in my Blog & it caused all the comments to get deleted so they had to re-post them...Perfectenschlag wrote: ↑Wed Oct 13, 2021 4:45 pm@Area1255_2021 - Do you have any explanation for why the only existing 3 positive reviews of your stack appeared within 6 minutes of each other, 5 days before you published your stack on your blog?Area1255_2021 wrote: ↑Mon Sep 13, 2021 1:23 pmThank You for linking these.facad2 wrote: ↑Mon Sep 13, 2021 12:07 pm
The comments were made here: https://area1255.blogspot.com/2014/11/i ... a.html?m=1
The stack post was made here: https://area1255.blogspot.com/2021/09/n ... d.html?m=1
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
When is it advice to stop the protocol after getting the benefits? 4 weeks?Area1255_2021 wrote: ↑Mon Oct 11, 2021 5:10 pmWell it takes a minimum of ~1 week to notice any differences, average is ~2 weeks, to notice any difference.flexstar13 wrote: ↑Mon Oct 11, 2021 4:00 pm I started with this stack on friday (without 9 me bc) and did not notice any changes yet.
A small percentage (maybe 4%) notice benefits to sexual function on the 3rd or 4th day. You've barely been on it 3 days, yet.
Will the benefits last after that?
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Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
~6 weeks, then the effects should last.Impermanence wrote: ↑Fri Oct 15, 2021 2:10 amWhen is it advice to stop the protocol after getting the benefits? 4 weeks?Area1255_2021 wrote: ↑Mon Oct 11, 2021 5:10 pmWell it takes a minimum of ~1 week to notice any differences, average is ~2 weeks, to notice any difference.flexstar13 wrote: ↑Mon Oct 11, 2021 4:00 pm I started with this stack on friday (without 9 me bc) and did not notice any changes yet.
A small percentage (maybe 4%) notice benefits to sexual function on the 3rd or 4th day. You've barely been on it 3 days, yet.
Will the benefits last after that?
Re: Nitric Oxide Stack to Treat PSSD (Cure PSSD Protocol 2021)
Area, apologies if you’ve already mentioned this somewhere else but have you personally been cured by this regimen?
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